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200 smooth muscle cell differentiation media 1x cell applications  (Cell Applications Inc)


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    Cell Applications Inc 200 smooth muscle cell differentiation media 1x cell applications
    200 Smooth Muscle Cell Differentiation Media 1x Cell Applications, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/200+smooth+muscle+cell+differentiation+media+1x+cell+applications/Human+Smooth+Muscle+Cell+Differentiation+Medium/pmc10039923__41536_2023_290_MOESM1_ESM-56-84-91
    Average 94 stars, based on 12 article reviews
    200 smooth muscle cell differentiation media 1x cell applications - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01

    Cell Differentiation:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01

    Electron Microscopy:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01

    Immunodetection:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01

    SYBR Green Assay:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01

    Magnetic Cell Separation:

    Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells
    Article Snippet: Antibodies Antibodies Anti-calponin 1:100 Abcam Ab46794 Anti-E-cadherin (2AE10) 1:100 Cell Signaling mAb#3195 Anti-Smooth muscle actin 1:200 Millipore Sigma A2547 Anti-Smooth muscle actin-Cy3 conjugate 1:100 Millipore Sigma C6198 Anti-Cytokeratin 5 1:500 Covance PRB-160P Cytokeratin-14 1:200 Covance PRB-155P Troma-III, Keratin 19 antibody 1:100 DSHB Troma-III-c conc Anti-NGF 1:200 Alomone Labs AN-240 Anti-Ngfr 1:100 LSBio B531 Anti-Tubb3 1:200 R&D MAB1195 Anti-TrkA 1:100 MyBiosource MBS5400171 Anti-TrkB 1:100 Cell signaling 80G2 Anti-Trk-C 1:100 Cell signaling C44H5 Anti-Mist1 1:250 Abcam ab187978 Anti-Nkcc1 1:250 Santa Cruz Biotechnology sc-21545 Anti-Cnn1 1:250 LSBio B4304 Anti-CollIV 1:100 EMD Millipore AB756P Anti-FGFR1 1:100 GeneTex GTX10646 Anti-FGFR1 1:100 Cell signaling 9470S Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-546-147 Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 705-606-147 Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-546-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 711-166-152 CyTM3 AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) 1:250 Jackson Immunoresearch Laboratories 715-165-150 Hoechst 1:1000 Thermo Fisher Scientific H3570 DAPI (Dihydrochloride) 1:10000 Millipore Sigma 268298 Supplementary Table 5. .. Chemicals, Peptides, and Recombinant Proteins DMEM-F12 1x Thermo Fisher Scientific 11320-082 Penicillin-streptomycin 1x Thermo Fisher Scientific 15140122 Recombinant human FGF10 protein 100 ng/mL R &D Systems Inc. 345-FG-025 Recombinant human EGF protein 20 ng/mL R &D Systems Inc 236-EG-200 Recombinant human FGF basic/FGF2 protein 20 ng/mL R &D Systems Inc 233-FB-025 Recombinant human SCF protein 100 ng/mL R &D Systems Inc 455-MC Recombinant mouse beta NGF protein 1-100 ng/mL R &D Systems Inc 1156-NG- 100 Smooth muscle cell growth media 1X Cell Applications, Inc. 311-200 Smooth muscle cell differentiation media 1X Cell Applications, Inc. 311D-250 N2 supplement 1X Thermo Fisher Scientific 17502001 Dexamethasone 1 mM Sigma Aldrich Corp D2915 Y27632- rock inhibitor 5 mM Sigma Aldrich Corp Y0503 Hyaluronidase from bovine testes 1 mg/mL Sigma Aldrich Corp H3506 Carbachol (Cch) 10 nM Sigma Aldrich Corp C4382 Collagenase Type II 0.575 mg/ml Thermo Fiscer Scientific 17101015 ITS-G 1X Thermo Fisher Scientific 41400045 Calcium chloride 6.25 mM Quality Biological 351-130-721 TrypLETM Express Enzyme (1X), phenol red 1X Thermo Fisher Scientific 12605036 Vitamin C 150 μg/mL Thermo Fisher Scientific 11107047 Transferrin 50 μg/mL Sigma Aldrich Corp A4544 GNF5837 R &D Systems Inc 4559/10 RO2750 R &D Systems Inc 2272/1 Dimethyl sulfoxide (DMSO) Millipore Sigma 34869-100ML Fluoro-Gel with TRIS mounting media Thermo Fisher Scientific 50-247-04 Paraformaldehyde (formaldehyde) Aqueous solution Electron Microscopy Sciences 15710 Xylene substitute Millipore Sigma A55971GAL Ethanol 100% Millipore Sigma E7023 Ethanol 95% Millipore Sigma 493538 Acetone Millipore Sigma 179973 Methanol Fisher Scientific A412-4 Critical Commercial Assays M.O.M (Mouse on Mouse) Immunodetection Kit Vector Laboratories MKB-2213-1 RNAqueous-4PCR kit and DNase removal reagent Thermo Fisher Scientific AM1914 RNAqueous Micro Kit Thermo Fisher Scientific AM1931 Superscript III First-Strand Synthesis System Life Technologies 18080051 iQ SYBR Green Supermix Bio-Rad 1708882 MACS SMART Strainers 70μm Miltenyi Biotech 130-110-916 24 well BioCoatTM Collagen IV multiwell plates Corning 08-774-29 8 well collagen IV coated 15-m slide Ibidi 50-305-885 15ml gentleMACS C tube Miltenyi Biotech 130-093-237 Corning® Costar® Ultra-Low Attachment Multiple Well Plate Millipore Sigma CLS3473 Whatman® NucleporeTM Track-Etched Membranes Millipore Sigma WHA110405 50 mm Dish, No. 1.5 Coverslip, 14 mm Glass Diameter, Uncoated MatTek P50G-1.514-F Supplementary Table 7. .. Software Beacon Designer PREMIER Biosoft N/A R & R studio https://rstudio.com/ N/A FIJI Schindelin et al. 2012 N/A Photoshop Adobe N/A Excel Microsoft N/A Word Microsoft N/A Powerpoint Microsoft N/A NDP.view2 Hakamatsu U12388-01



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